Anna's training mode
Select story mode and successfully complete all of Anna's training.

Sudden death mode
Successfully complete story mode under the normal or higher difficulty setting.

Bonus characters
Successfully complete story mode under the normal or higher difficulty setting using various characters to unlock Over Soul 1 Yoh, Over Soul 2 Yoh, Silva, Horo Horo, Faulst, Maruko, and others.

Play as Anna
Beat the high score in each mini-game in Anna's training mode.

Play as Choco Love
Defeat Choco Love during story mode then complete story mode.

Play as Faust VIII
Defeat Faust VIII during story mode then complete story mode.

Play as Horo Horo
Defeat Horo Horo during story mode then complete story mode.

Play as Iron Maiden Jeanne
Unlocking all other characters, then successfully complete Shaman fight mode as Maruko under the normal or higher difficulty setting.

Play as Malco
Defeat Malco during story mode then complete story mode.

Play as Manta
Successfully complete sudden death mode as Anna under the normal or higher difficulty setting.

Play as Reserg
Defeat Reserg during story mode then complete story mode.

Play as Silver
Defeat Silver during story mode then complete story mode.

Play as Tamao
Successfully complete Shaman fight mode as Over Soul 1 Yoh under the normal or higher difficulty setting.

Play as Yoh 2
Successfully complete the game in story mode.

Play as Yoh 3
Successfully complete the game in story mode and complete cave training.

Play as Youmei
Successfully complete sudden death mode as Original Yoh under the normal or higher difficulty setting.